25 – 26 September

London, United Kingdom

 

Day One
Monday 25th September, 2017

Day Two
Tuesday 26th September, 2017

08.00
Registration & Coffee

09.00
Chair’s Opening Remarks

09.10
Deciphering the DNA Repair Mechanisms Involved in CRISPR-Induced Gene Targeting: Focus on the Model Physcomitrella Patens

  • Cécile Collonnier Academic Scientist, , French National Institute for Research in Agronomy (INRA)

Synopsis

  • P. patens, an excellent model to study DNA repair pathways involved in CRISPR-mediated gene targeting in plants
  • Identification of key factors involved in CRISPR-induced gene knock-in in plants
  • Increasing CRISPR-induced gene knock-in frequency and quality by orienting DNA repair pathways in the cells

09.40
CRISPR/Cas9 Based Gene Editing in Barley & Potato: From Construct Design to Mutation Screening

  • Abdellah Barakate Post-Doctoral Researcher, Molecular Geneticist, The James Hutton Institute, The University of Dundee

Synopsis

  • Improvements of CRISPR/Cas9 gene editing protocol from construct design to mutation (Indels) screening
  • Use of CRISPR/Cas9 to manipulate meiosis, lignin and other pathways in barley, wheat and potato
  • Highlight encountered problems and solutions including failed experiments
  • Attempts to improve gene replacement efficiency

10.10
CRISPR/Cas9 Genome Editing in UK Crops

  • Wendy Harwood Professor & Senior Scientist, Crop Genetics Department, John Innes Centre

Synopsis

  • The production of targeted gene knock-outs is impacting many different areas of research
  • Examples will be given in wheat, barley and Brassica crops and the latest developments in the technologies discussed
  • Technologies to increase throughput and efficiency will be described
  • A platform has been established to provide this technology to the research community

10.40
Speed Networking & Morning Refreshments

11.40
CRISPR Cas9 & CRISPR Cpf1 to Manipulate Stomatal Developmental Pathway in Rice

Synopsis

  • Modification of CRISPR Cas9 and Cpf1 for application in rice
  • Targeting of genes related to stomatal developmental pathway
  • Alteration of stomatal number in rice and impact on WUE (water use efficiency)

Advancing the Application of Precision Gene Editing in Agricultural R&D

12.10
Harnessing CRISPR/Cas9 for Functional Genomics in Trait Discovery & Selection

Synopsis

  • Optimising CRISPR multiplexing to study gene interactions
  • Investigating the role of genes in disease resistance
  • Applications of gene editing in functional genomics and trait discovery

12.40
Networking Lunch

13.40
A Dual-sgRNA Approach for Functional Genomics in Arabidopsis Thaliana

  • Laurens Pauwels Project Leader, VIB Center for Plant Systems Biology, Ghent University

Synopsis

  • Efficient generation of inheritable mutations in Arabidopsis using CRISPR/Cas9
  • Deleting gene fragments by a paired nuclease approach to obtain knockouts
  • Examples of applications in tomato and corn to study genes implicated in leaf development

14.10
Opportunities and Challenges of Directed Mutagenesis in Crops

  • Jan Schaart Scientific Researcher, Wageningen Universiteit

Synopsis

  • Application of Zinc Finger Nuclease and CRISPR-Cas for mutagenesis in different crops
  • Induction of multi-allelic deletion in polyploids
  • Tissue culture solutions

14.40
Afternoon Refreshments & Poster Session

15.10
Interactive Workshop: Optimisation of CRISPR/Cas9 Specificity, Efficiency & Delivery in Plants

Synopsis

This workshop will help you maximise the use of CRISPR in your research through case study-driven discussions on optimising CRISPR application in plants and crops.
You will learn the following lessons in this interactive workshop:

  • Pros and cons of CRISPR/Cas9 variants and Cpf1 in off-target efficiencies
  • Improving off-target detection in plants and crops
  • Optimising homology-directed repair (HDR) opposed to non-homologous directed repair for gene replacement
  • Utilising the gene gun (biolistics) method
  • Developing agrobacterium mediated delivery into plants
  • Delivery of RNPs in plant
  • Nano material cargos for delivering CRISPR-Cas systems in nucleus and chloroplast